Who we are

Welcome to Marcelo Nollmann's research group at the Center for Structural Biochemistry of the CNRS/INSERM

We develop single-molecule and advanced microscopy methodologies to understand the mechanisms underlying DNA organization and transcription in multi-cellular organisms.

Click here for links to our multiplexed FISH (Hi-M) acquisition (qudi-CBS) and analysis (pyHiM) software packages.

News

• Congrats Olivier for submitting his manuscript entitled "Chromatin folding motifs define the basic grammar of TAD architecture in single alleles" to the BioRxiv, available as a biorxiv preprint.
• Congrats Xavier and JB on the publication of their work describing pyHiM, an open-source package to analyze multiplexed DNA-FISH data, in Genome Biology, available here.
• Congrats Olivier on his paper being accepted by Nature Communications and available as a biorxiv preprint.
• Congratulations Sara, Anna and Antoine on their paper at Nature Communications published this last september (see here) and available as a biorxiv preprint.
• pyHiM: our soft for Hi-M analysis is available at GitHub (see here) and the bioRxiv ms available here.
• qudiHiM: our soft for HiM acquisition GitHub (see here) and the paper available
• We are happy to present our latest work on 3DGenome organization and transcription during drosophila development, published in Nature Genetics on Apr 1/21 (see here) and available as a biorxiv preprint.
• Want to know how phase-separated droplets are segregated in bacteria? See our new article in Molecular Cell! check out the biorxivpreprint and the paper in Molecular Cell. Voir aussi publication du CNRS.
• Do you want to do Hi-M in your lab? Check out our VERY detailed protocol in Nature Protocols here.
• Nov'19: Prix Etancelin de l'Academie des Sciences See here
• Apr 19: Congratulations Jean-Bernard and Antoine for their papers appearing in Science and Nanoscale!
• Feb 19: congratulations to the team for the Hi-M paper being accepted in Mol Cell !
• Jan 18: physics to understand living systems: interview by the Bettencourt Schueller Fondation.
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Featured Publications

pyHiM: a new open-source, multi-platform software package for spatial genomics based on multiplexed DNA-FISH imaging
Devos, et al., Genome Biology, 2024, 25:47.

Multiplexed chromatin imaging reveals predominantly pairwise long-range coordination between Drosophila Polycomb genes
Gurgo, et al., Cell Reports, 2024, 43(5):114167

Multiple parameters shape the 3D chromatin structure of single nuclei
Goetz, et al., Nature Communications, 2022, 13:5375.

3D chromatin interactions involving Drosophila insulators are infrequent but preferential and arise before TADs and transcription
Messina, et al., Nature Communications 2023, 14, 6678.

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Cis-regulatory chromatin loops arise before TADs and gene activation, and are independent of cell fate during development
Espinola*, Götz*, et al. Nature Genetics (2021)

ATP-Driven Separation of Liquid Phase Condensates in Bacteria
Guilhas, et al, Mol Cell (2020)

Microscopy-based chromosome conformation capture enables simultaneous visualization of genome organization and transcription in intact organisms
​Cardozo-Gizzi, et al. Mol Cell (2019)

Direct and simultaneous observation of transcription and chromosome architecture in single cells with Hi-M.
​Cardozo-Gizzi, et al. Nature Protocols (2020)

The mechanism of force transmission at bacterial focal adhesion complexes.
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Faure, et al. Nature (2016) [Blog] [Video]

Single-cell absolute contact probability detection reveals that chromosomes are organized by multiple low-frequency yet specific interaction
Cattoni, et al. Nature Comm (2017)

Bacterial partition complexes segregate within the volume of the nucleoid
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Le Gall, et al. Nature Comm (2016)

Condensin- and replication-mediated dynamic folding of a bacterial chromosome and its origin domain revealed by super-resolution imaging/ Hi-C​
​Marbouty, Le Gall, et al. Mol Cell (2015)

Funders

Sponsors

Thanks Zulip for sponsoring our lab!