Who we are

Welcome to Marcelo Nollmann's research group at the Center for Structural Biochemistry of the CNRS/INSERM

We develop single-molecule and advanced microscopy methodologies to understand the mechanisms underlying DNA organization and transcription in multi-cellular organisms.

News

• We are happy to present our latest work on 3DGenome organization and transcription during drosophila development, now available as a biorxivpreprint.
• Want to know how phase-separated droplets are segregated in bacteria? See our new article in Molecular Cell! check out the biorxivpreprint
• Do you want to do Hi-M in your lab? Check out our VERY detailed protocol in Nature Protocols here.
• Nov'19: Prix Etancelin de l'Academie des Sciences See here
• Apr 19: Congratulations Jean-Bernard and Antoine for their papers appearing in Science and Nanoscale!
• Feb 19: congratulations to the team for the Hi-M paper being accepted in Mol Cell !
• Jan 18: physics to understand living systems: interview by the Bettencourt Schueller Fondation.

Featured Publications

We developed a unique method to visualise chromosome architecture and transcriptional status in single cells within developing drosophila embryos
​
​Cardozo-Gizzi, et al. Mol Cell (2019)

---

Isotropic angular reconstruction from 2D super-resolution images

​We worked out a method to adapt cryo-EM reconstruction algorithms to obtain isotropic 3D structures from 2D super-resolution images

​Salas, Le Gall, et al, PNAS (2017) [PDF]
Highlights in Nat Methods [PDF]

Molecular dissection of bacterial gliding

We deciphered the mechanism by which  Myxococcus Xhantus glide on surfaces using a revolutionary combination of advanced microscopies and microfluidics.​
​​
​ Faure, et al. Nature (2016)
[Blog] [Video]

Bacterial chromosome structure

Condensin- and replication-mediated dynamic folding of a bacterial chromosome and its origin domain revealed by super-resolution imaging and Hi-C​

​Marbouty, et al. Mol Cell (2015)

TAD organization is not stochastic!

 We used microscopy to measure the absolute contact probabilities between genomic loci. See more here!
​
​
Cattoni, Cardozo-Gizzi, Georgieva, et al, Nature Comm (2017)​​ [PDF]

Plasmid segregation super-resolved

We used super-resolution and multifocus microscopies to show that plasmids segregate through bacterial nucleoids by the action of ParA  and by relying on the host chromosome scaffold​
​Le Gall, et al. Nature Comm (2016)

A complete list of publications, including chapters and articles not accessed by PubMed, can be found here.

Funders