Our current research projects
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DNA organization and segregation in bacteriaChromosomes are hierarchically organized in the cell. This organization impacts and is impacted by several DNA transactions, such as DNA replication and transcription. We are interested in understanding the interplay between chromosome structure and its possible functional roles. We use super-resolution, advanced microscopies, and Hi-C to investigate these issues in bacteria (B. subtilis and E. coli) and in eukaryotes (Drosophila Melanogaster).
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Molecular MotorsDNA motors use the enerygy of ATP hydrolysis to processively translocate DNA between cellular compartments or within different cells, and belong to the large and diverse AAA+ family of enzymes. AAA+ motors form multimeric rings in which ATP hydrolysis and mechanical work are closely coupled. In general, we are interested in understanding the larger network of genetic interactions affecting the process of DNA transport in vivo. In particular, we want to understand the general mechanisms governing translocation directionality, transduction of energy into movement, and complex assembly
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Single-molecule and super-resolution microscopiesStudying these problems requires the continued development of new technologies. In our lab, we concentrate in super-resolution, and single-molecule-based microscopies as well as in data analysis of complex datasets from microscopy or genome-wide sources.
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